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100+ results found
  1. R

    Asparagine N-linked glycosylation

    • reactome.org
  2. R

    Data from: Diseases of glycosylation

    • reactome.org
  3. R

    O-linked glycosylation

    • reactome.org
  4. GWAS summary statistics for UPLC IgG N-glycosylation traits

    • datashare.is.ed.ac.uk
    • search.datacite.org
    Published Dec 21, 2018
  5. R

    Diseases associated with O-glycosylation of proteins

    • reactome.org
  6. f

    Data from: Large-Scale Measurement of Absolute Protein Glycosylation...

    • figshare.com
    Updated Feb 15, 2016
  7. f

    Centralized Modularity of N-Linked Glycosylation Pathways in Mammalian Cells...

    • figshare.com
    Updated Jan 18, 2016
  8. o

    Deconstruction of protein O-glycosylation

    • www.omicsdi.org
  9. s

    O-GLYCBASE

    • scicrunch.org
  10. f

    Data from: Large-scale Identification of N-Glycosylated Proteins of Mouse...

    • figshare.com
    Updated Feb 20, 2016
  11. Separation and characterization of the two Asn-linked glycosylation sites of...

    • researchdata.ands.org.au
    Published Jun 26, 2012
  12. o

    Data from: The metacaspase Mca1p restricts O-glycosylation during...

    • www.omicsdi.org
  13. o

    To identify glycosylation-specific targets contributing to the interferon...

    • omictools.com
    • www.omicsdi.org
    • +1more
    Updated Apr 20, 2017
  14. f

    Data from: Comprehensive Analysis of Protein N‑Glycosylation Sites by...

    • figshare.com
    Updated Feb 18, 2016
  15. Dataset pertaining to the publication “Loci Associated with N-Glycosylation...

    • datashare.is.ed.ac.uk
    • search.datacite.org
    Published Apr 12, 2016
  16. o

    Proteomics of Campylobacter glycosylation

    • www.omicsdi.org
  17. o

    Irani2015 - Genome-scale metabolic model of P.pastoris N-glycosylation

    • www.omicsdi.org
  18. r

    Acetonitrile in Glycosylation

    • www.researchgate.net
    Published Jan 1, 2004
  19. The glycosylation and structure of human serum IgA1, Fab, and Fc regions and...

    • researchdata.ands.org.au
    Published Jun 26, 2012
  20. o

    Data from: Quantitative Profiling of N-linked Glycosylation Machinery in...

    • www.omicsdi.org
  21. o

    Elucidating mechanisms by which regulated expression of proteoglycans and...

    • omictools.com
    • www.omicsdi.org
    Updated Mar 23, 2012
  22. t

    BIOGRID CURATED DATA FOR PUBLICATION: Glycosylation and stabilization of...

    • thebiogrid.org
  23. R

    N-linked glycosylation of WNTs

    • reactome.org
  24. o

    Pilot project to determine optimal bioreactor protein glycosylation...

    • omictools.com
    Updated Sep 5, 2014
  25. r

    Data from: Glycosylation of TRPM4 and TRPM5 Channels: Molecular Determinants...

    • www.researchgate.net
    Published Feb 24, 2014
  26. w

    Effects of Glycosylation on the Structure and Dynamics of Eel Calcitonin in...

    • www.bmrb.wisc.edu
    • bmrb.cerm.unifi.it
    Updated Dec 3, 1999
  27. Data from: O- and N-glycosylation of the Leishmania mexicana-secreted acid...

    • researchdata.ands.org.au
    Published Jun 26, 2012
  28. f

    N-glycosylation sites of Phlebotomus tobbi salivary proteins.

    • figshare.com
    Updated Dec 2, 2015
  29. s

    GlySeq

    • scicrunch.org
  30. w

    Effects of Glycosylation on the Structure and Dynamics of Eel Calcitonin

    • www.bmrb.wisc.edu
    • bmrb.cerm.unifi.it
    • +1more
    Updated Jul 13, 2007
  31. w

    truncated AcrA from Campylobacter jejuni for glycosylation studies

    • www.bmrb.wisc.edu
    • bmrb.cerm.unifi.it
    • +1more
    Updated Jun 2, 2010
  32. o

    The role of glycans and glycosylation in cell-type specific effects on viral...

    • omictools.com
    • www.omicsdi.org
    Updated Mar 23, 2012
  33. O-glycosylation of TSR domain-containing proteins

    • reactome.org
  34. o

    Glycosylation of the HA protein of H5N1 virus increases its virulence in...

    • omictools.com
    • www.omicsdi.org
  35. t

    BIOGRID CURATED DATA FOR PUBLICATION: Disruption of N-linked glycosylation...

    • thebiogrid.org
  36. f

    Data from: Evaluation of Different N‑Glycopeptide Enrichment Methods for...

    • figshare.com
    Updated Aug 29, 2016
  37. o

    Glycosylation analysis of TNF-alpha-induced insulin resistance

    • www.omicsdi.org
  38. s

    PTMcode

    • scicrunch.org
  39. o

    The use of the glycoarray chip to investigate VHL-dependent protein...

    • www.omicsdi.org
    • datamed.org
  40. t

    BIOGRID CURATED DATA FOR PUBLICATION: Pim-1 kinase protects P-glycoprotein...

    • thebiogrid.org
  41. o

    Data from: Topological N-glycosylation and site-specific N-glycan sulfation...

    • www.omicsdi.org
  42. a

    ALG12

    • www.alliancegenome.org
    Updated Jun 8, 2019
  43. a

    ALG10B

    • www.alliancegenome.org
    Updated Jun 8, 2019
  44. f

    Data from: Deciphering Protein O‑Glycosylation: Solid-Phase Chemoenzymatic...

    • figshare.com
    Updated Jun 24, 2018
  45. o

    Data from: Detecting Proteins Glycosylation by a Homogeneous Reac-tion...

    • www.omicsdi.org
  46. o

    Data from: RNA-Seq analysis of glycosylation related gene expression in...

    • omictools.com
    • www.omicsdi.org
    • +1more
    Updated Jun 28, 2018
  47. Data from: Novel N-glycosylation in eukaryotes: laminin contains the linkage...

    • researchdata.ands.org.au
    Published Jun 26, 2012
  48. o

    N-linked glycosylation enrichment for in-depth cell surface proteomics and...

    • www.omicsdi.org
  49. o

    To understand how glycosylation on human B cells is regulated during...

    • omictools.com
    • datamed.org
    Updated Apr 20, 2017
  50. r

    Data from: Expression and purification of non-N-glycosylated porcine...

    • www.researchgate.net
    Published Mar 1, 2012
  51. o

    A conserved atypical MFS family member orchestrates a subset of...

    • www.omicsdi.org
  52. o

    Data from: Death receptor O-glycosylation controls tumor-cell sensitivity to...

    • omictools.com
    • www.omicsdi.org
    Updated Oct 29, 2018
  53. d

    LYPD8_MOUSE

    • datamed.org
  54. R

    Defective SLC35A2 causes congenital disorder of glycosylation 2M (CDG2M)

    • reactome.org
  55. d

    Coordinate Regulation of Metabolites Glycosylation and Stress Hormones...

    • datamed.org
    • omictools.com
  56. r

    S3 Fig

    • www.researchgate.net
    Published Jun 9, 2016
  57. r

    Figure S2

    • www.researchgate.net
    Published Jun 10, 2011
  58. R

    Asparagine N-linked glycosylation

    • reactome.org
  59. f

    Data from: Identification of Glycopeptides with Multiple Hydroxylysine...

    • figshare.com
    Updated Feb 12, 2016
  60. m

    Sialylation of OprD hinders antibiotic entry

    • data.mendeley.com
    • search.datacite.org
    Updated Sep 28, 2018
  61. Data from: Glycosylation sites and site-specific glycosylation in human...

    • researchdata.ands.org.au
    Published Jun 26, 2012
  62. o

    Data from: N-glycosylation Profiling of Colorectal Cancer Cell Lines Reveals...

    • www.omicsdi.org
  63. Data from: Phosphorylation and O-glycosylation sites of human chromogranin A...

    • researchdata.ands.org.au
    Published Jun 26, 2012
  64. a

    Alg10

    • www.alliancegenome.org
    Updated Jun 8, 2019
  65. r

    Figure S7

    • www.researchgate.net
    Published Mar 16, 2012
  66. d

    Autosomal recessive PGM3 mutations cause a new Congenital Disorder of...

    • datamed.org
  67. a

    GALNT4

    • www.alliancegenome.org
    Updated Jun 8, 2019
  68. f

    Influence of Glycosylation Inhibition on the Binding of KIR3DL1 to...

    • figshare.com
    Updated Jan 15, 2016
  69. d

    How an ABO-incompatible graft may be protected by phenotype-specific...

    • search.datacite.org
    Updated Jun 7, 2016
  70. m

    Ddost

    • rgd.mcw.edu
  71. d

    How an ABO-incomcatible graft may survive by phenotype-specific...

    • search.datacite.org
    Updated May 23, 2016
  72. R

    O-linked glycosylation of mucins

    • reactome.org
  73. d

    Expression data from Congenital disorders of Glycosylation type-1 patients...

    • datamed.org
  74. o

    Identification of human gene expression induced by 4MU-xyloside treatment

    • omictools.com
    • www.omicsdi.org
    Updated Sep 15, 2018
  75. R

    Diseases associated with N-glycosylation of proteins

    • reactome.org
  76. a

    TMEM165

    • www.alliancegenome.org
    Updated Jun 8, 2019
  77. f

    GlycoProtDB

    • fairsharing.org
  78. N-linked glycosylation of native and recombinant cauliflower xyloglucan...

    • researchdata.ands.org.au
    Published Jun 26, 2012
  79. a

    The significance of glycosylation on the function of the calcium-sensing...

    • researchdata.ands.org.au
    Published Sep 20, 2012
  80. r

    Figure S2

    • www.researchgate.net
    Published Aug 30, 2011
  81. a

    Data from: O-linked glycosylation of rat renal gamma-glutamyltranspeptidase...

    • researchdata.ands.org.au
    Published Jun 26, 2012
  82. Data from: Retinal GlcNAc-transferases and the glycosylation of rhodopsin.

    • researchdata.ands.org.au
    Published Jun 26, 2012
  83. f

    Data from: Mapping N-Linked Glycosylation Sites in the Secretome and Whole...

    • figshare.com
    Updated Feb 22, 2016
  84. f

    Potential glycosylation sites and solvent accessibility values of spectrinN....

    • figshare.com
    Updated Dec 2, 2015
  85. a

    TUSC3

    • www.alliancegenome.org
    Updated Jun 8, 2019
  86. o

    Microarray analysis of RNA of TCR transgenic CD8 T cells to assess the role...

    • omictools.com
    • www.omicsdi.org
    • +1more
    Updated Mar 23, 2012
  87. o

    Gene Expression in NS0 cells under ammonium stress

    • omictools.com
    • www.omicsdi.org
    Updated Mar 21, 2012
  88. m

    Mgat2

    • rgd.mcw.edu
  89. r

    Figure S4

    • www.researchgate.net
    Published Apr 18, 2012
  90. o

    Data from: Site-specific O-Glycosylation Analysis of Human Blood Plasma...

    • www.omicsdi.org
  91. r

    Data from: Figure S3

    • www.researchgate.net
    Published Feb 23, 2011
  92. o

    Data from: The secretome and N-glycosylation profiles of the charophycean...

    • www.omicsdi.org
  93. Data from: Glycosylation of mammalian neurofilaments. Localization of...

    • researchdata.ands.org.au
    Published Jun 26, 2012
  94. f

    Data from: Analysis of Congenital Disorder of Glycosylation-Id in a Yeast...

    • figshare.com
    Updated Feb 20, 2016
  95. m

    Ddost

    • rgd.mcw.edu
  96. a

    ALG10

    • www.alliancegenome.org
    Updated Jun 8, 2019
  97. o

    Identification of human genes of which expression are induced by...

    • omictools.com
    Updated Aug 2, 2018
  98. a

    St3gal1

    • www.alliancegenome.org
    Updated May 29, 2019
  99. o

    Data from: Quantitative proteomics reveals the effect of protein...

    • www.omicsdi.org
  100. s

    GlyVicinity

    • scicrunch.org
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Asparagine N-linked glycosylation

301 scholarly articles cite this dataset (View in Google Scholar)
Authors
Giovanni Dall'Olio
Available download formats from providers
biopax2, pdf, sbgn, biopax3, owl, sbml, docx
Description

N-linked glycosylation is the most important form of post-translational modification for proteins synthesized and folded in the Endoplasmic Reticulum (Stanley et al. 2009). An early study in 1999 revealed that about 50% of the proteins in the Swiss-Prot database at the time were N-glycosylated (Apweiler et al. 1999). It is now established that the majority of the proteins in the secretory pathway require glycosylation in order to achieve proper folding.
The addition of an N-glycan to a protein can have several roles (Shental-Bechor & Levy 2009). First, glycans enhance the solubility and stability of the proteins in the ER, the golgi and on the outside of the cell membrane, where the composition of the medium is strongly hydrophilic and where proteins, that are mostly hydrophobic, have difficulty folding properly. Second, N-glycans are used as signal molecules during the folding and transport process of the protein: they have the role of labels to determine when a protein must interact with a chaperon, be transported to the golgi, or targeted for degradation in case of major folding defects. Third, and most importantly, N-glycans on completely folded proteins are involved in a wide range of processes: they help determine the specificity of membrane receptors in innate immunity or in cell-to-cell interactions, they can change the properties of hormones and secreted proteins, or of the proteins in the vesicular system inside the cell.
All N-linked glycans are derived from a common 14-sugar oligosaccharide synthesized in the ER, which is attached co-translationally to a protein while this is being translated inside the reticulum. The process of the synthesis of this glycan, known as Synthesis of the N-glycan precursor or LLO, constitutes one of the most conserved pathways in eukaryotes, and has been also observed in some eubacteria. The attachment usually happens on an asparagine residue within the consensus sequence asparagine-X-threonine by an complex called oligosaccharyl transferase (OST).
After being attached to an unfolded protein, the glycan is used as a label molecule in the folding process (also known as Calnexin/Calreticulin cycle) (Lederkremer 2009). The majority of the glycoproteins in the ER require at least one glycosylated residue in order to achieve proper folding, even if it has been shown that a smaller portion of the proteins in the ER can be folded without this modification.
Once the glycoprotein has achieved proper folding, it is transported via the cis-Golgi through all the Golgi compartments, where the glycan is further modified according to the properties of the glycoprotein. This process involves relatively few enzymes but due to its combinatorial nature, can lead to several millions of different possible modifications. The exact topography of this network of reactions has not been established yet, representing one of the major challenges after the sequencing of the human genome (Hossler et al. 2006).
Since N-glycosylation is involved in an great number of different processes, from cell-cell interaction to folding control, mutations in one of the genes involved in glycan assembly and/or modification can lead to severe development problems (often affecting the central nervous system). All the diseases in genes involved in glycosylation are collectively known as Congenital Disorders of Glycosylation (CDG) (Sparks et al. 2003), and classified as CDG type I for the genes in the LLO synthesis pathway, and CDG type II for the others.

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