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100+ results found
  1. R

    Asparagine N-linked glycosylation

    • reactome.org
  2. a

    The significance of glycosylation on the function of the calcium-sensing...

    • researchdata.ands.org.au
    Published Sep 20, 2012
  3. O-linked glycosylation of mucins

    • reactome.org
  4. Data from: Diseases of glycosylation

    • reactome.org
  5. R

    N-linked glycosylation of WNTs

    • reactome.org
  6. Diseases associated with N-glycosylation of proteins

    • reactome.org
  7. R

    Glycosylation of Hh

    • reactome.org
  8. Diseases associated with O-glycosylation of proteins

    • reactome.org
  9. O-linked glycosylation

    • reactome.org
  10. Defective SLC35A2 causes congenital disorder of glycosylation 2M (CDG2M)

    • reactome.org
  11. O-glycosylation of TSR domain-containing proteins

    • reactome.org
  12. Glycosylation of NA

    • reactome.org
  13. R

    O-linked glycosylation

    • reactome.org
  14. R

    Asparagine N-linked glycosylation

    • reactome.org
  15. Glycosylation of Pre-NOTCH by FRINGE

    • reactome.org
  16. R

    O-linked glycosylation of mucins

    • reactome.org
  17. R

    Glycosylation of ENV polyprotein

    • reactome.org
  18. Glycosylation and Folding of HA

    • reactome.org
  19. f

    Data from: Large-scale Identification of N-Glycosylated Proteins of Mouse...

    • figshare.com
    Updated Feb 20, 2016
  20. f

    MOESM1 of N-glycosylation proteome enrichment analysis in kidney reveals...

    • figshare.com
    Updated Dec 15, 2016
  21. f

    Data from: Mapping N-Linked Glycosylation Sites in the Secretome and Whole...

    • figshare.com
    Updated Feb 22, 2016
  22. o

    Data from: N-glycosylation Profiling of Colorectal Cancer Cell Lines Reveals...

    • www.omicsdi.org
  23. f

    Data from: Deciphering Protein O‑Glycosylation: Solid-Phase Chemoenzymatic...

    • figshare.com
    Updated Jun 24, 2018
  24. R

    Glycosylation of mouse WNTs

    • reactome.org
  25. o

    Data from: The metacaspase Mca1p restricts O-glycosylation during...

    • www.omicsdi.org
  26. f

    Data from: Targeted Mass Spectrometry Approach Enabled Discovery of...

    • figshare.com
    Updated Aug 7, 2017
  27. o

    To identify glycosylation-specific targets contributing to the interferon...

    • www.omicsdi.org
    • omictools.com
  28. o

    Data from: Detecting Proteins Glycosylation by a Homogeneous Reac-tion...

    • www.omicsdi.org
  29. R

    Glycosylation of Hh

    • reactome.org
  30. f

    Data from: Organocatalyzed Direct Glycosylation of Unprotected and...

    • figshare.com
    Updated Feb 18, 2016
  31. Glycosylation of Pre-Notch by Fringe

    • reactome.org
  32. f

    Data from: A New Method for the Rapid Diagnosis of Protein N‑linked...

    • figshare.com
    Updated Feb 19, 2016
  33. o

    The role of glycans and glycosylation in cell-type specific effects on viral...

    • www.omicsdi.org
    • omictools.com
  34. o

    Deconstruction of protein O-glycosylation

    • www.omicsdi.org
  35. f

    GlycoProtDB

    • fairsharing.org
  36. o

    Data from: Topological N-glycosylation and site-specific N-glycan sulfation...

    • www.omicsdi.org
  37. a

    Data from: Separation and characterization of the two Asn-linked...

    • researchdata.ands.org.au
    Published Jun 26, 2012
  38. Defective SLC35A1 causes congenital disorder of glycosylation 2F (CDG2F)

    • reactome.org
  39. f

    Surface Glycosylation Profiles of Urine Extracellular Vesicles

    • figshare.com
    Updated Dec 2, 2015
  40. o

    Data from: Site-specific O-Glycosylation Analysis of Human Blood Plasma...

    • www.omicsdi.org
  41. o

    Irani2015 - Genome-scale metabolic model of P.pastoris N-glycosylation

    • www.omicsdi.org
  42. Dataset pertaining to the publication “Loci Associated with N-Glycosylation...

    • datashare.is.ed.ac.uk
    Published Apr 12, 2016
  43. o

    N-linked glycosylation enrichment for in-depth cell surface proteomics and...

    • www.omicsdi.org
  44. s

    O-GLYCBASE

    • scicrunch.org
  45. f

    Data from: Surface-exposed Glycoproteins of Hyperthermophilic Sulfolobus...

    • figshare.com
    Updated Feb 19, 2016
  46. o

    The use of the glycoarray chip to investigate VHL-dependent protein...

    • www.omicsdi.org
    • datamed.org
  47. f

    Data from: Exploring Site-Specific N‑Glycosylation Microheterogeneity of...

    • figshare.com
    Updated Feb 19, 2016
  48. f

    Data from: Specific and Nonspecific Effects of Glycosylation

    • figshare.com
    Updated Feb 20, 2016
  49. a

    The glycosylation and structure of human serum IgA1, Fab, and Fc regions and...

    • researchdata.ands.org.au
    Published Jun 26, 2012
  50. R

    N-glycan trimming in the ER and Calnexin/Calreticulin cycle

    • reactome.org
  51. a

    congenital disorder of glycosylation type II

    • stage.alliancegenome.org
  52. a

    Data from: O- and N-glycosylation of the Leishmania mexicana-secreted acid...

    • researchdata.ands.org.au
    Published Jun 26, 2012
  53. a

    Data from: N-glycosylation of horseradish peroxidase from cell culture.

    • researchdata.ands.org.au
    Published Jun 26, 2012
  54. a

    Data from: Characterization of the glycosylation on recombinant human...

    • researchdata.ands.org.au
    Published Jun 26, 2012
  55. a

    Data from: Glycosylation pattern and processing of envelope gene products...

    • researchdata.ands.org.au
    Published Jun 26, 2012
  56. o

    Data from: RNA-Seq analysis of glycosylation related gene expression in...

    • www.omicsdi.org
    • omictools.com
    • +1more
  57. a

    Data from: Drastic differences in glycosylation of related S-layer...

    • researchdata.ands.org.au
    Published Jun 26, 2012
  58. a

    Data from: Microheterogeneity of N-glycosylation on a stylar...

    • researchdata.ands.org.au
    Published Jun 26, 2012
  59. a

    Data from: Characterization of the glycosylation sites in cyclooxygenase-2...

    • researchdata.ands.org.au
    Published Jun 26, 2012
  60. o

    A conserved atypical MFS family member orchestrates a subset of...

    • www.omicsdi.org
  61. o

    Data from: Quantitative proteomics reveals the effect of protein...

    • www.omicsdi.org
  62. a

    Data from: Glycosylation of the envelope glycoprotein from a polytropic...

    • researchdata.ands.org.au
    Published Jun 26, 2012
  63. a

    ALG10B

    • stage.alliancegenome.org
    Updated Feb 18, 2019
  64. a

    Data from: Glycosylation of glycoprotein 55 encoded by the anaemia-inducing...

    • researchdata.ands.org.au
    Published Jun 26, 2012
  65. f

    Data from: Integrity of Glycosylation Processing of a...

    • figshare.com
    Updated Feb 8, 2018
  66. o

    Elucidating mechanisms by which regulated expression of proteoglycans and...

    • www.omicsdi.org
    • omictools.com
  67. a

    Data from: Retinal GlcNAc-transferases and the glycosylation of rhodopsin.

    • researchdata.ands.org.au
    Published Jun 26, 2012
  68. f

    Data from: High-Throughput Profiling of Protein N‑Glycosylation by...

    • figshare.com
    Updated Feb 17, 2016
  69. o

    Data from: Site-specific glycosylation of donkey milk lactoferrin...

    • www.omicsdi.org
  70. f

    MOESM2 of Mass spectrometry-based analyses showing the effects of secretor...

    • figshare.com
    Updated Dec 14, 2016
  71. a

    Data from: Change in glycosylation of chicken transferrin glycans...

    • researchdata.ands.org.au
    Published Jun 26, 2012
  72. a

    Data from: Protein N-glycosylation is similar in the moss Physcomitrella...

    • researchdata.ands.org.au
    Published Jun 26, 2012
  73. s

    GlySeq

    • scicrunch.org
  74. Defective MOGS causes MOGS-CDG (CDG-2b)

    • reactome.org
  75. o

    Autosomal recessive PGM3 mutations cause a new Congenital Disorder of...

    • www.omicsdi.org
  76. a

    Data from: Novel N-glycosylation in eukaryotes: laminin contains the linkage...

    • researchdata.ands.org.au
    Published Jun 26, 2012
  77. o

    Coordinate Regulation of Metabolites Glycosylation and Stress Hormones...

    • www.omicsdi.org
  78. a

    Data from: The glycosylation of Bowes melanoma tissue plasminogen activator:...

    • researchdata.ands.org.au
    Published Jun 26, 2012
  79. d

    Expression data from Congenital disorders of Glycosylation type-1 patients...

    • datamed.org
  80. s

    PTMcode

    • scicrunch.org
  81. a

    Data from: The conformational effects of N-glycosylation on the tailpiece...

    • researchdata.ands.org.au
    Published Jun 26, 2012
  82. a

    Data from: O-linked glycosylation of rat renal gamma-glutamyltranspeptidase...

    • researchdata.ands.org.au
    Published Jun 26, 2012
  83. a

    N-linked glycosylation of native and recombinant cauliflower xyloglucan...

    • researchdata.ands.org.au
    Published Jun 26, 2012
  84. f

    Data from: IgA Nephropathy Caused by Unusual Polymerization of IgA1 with...

    • figshare.com
    Updated Jan 18, 2016
  85. d

    LYPD8_MOUSE

    • datamed.org
  86. f

    Additional file 2: Table S1. of Altered glycosylation of exported proteins,...

    • figshare.com
    Updated Dec 14, 2016
  87. a

    Data from: Glycosylation of mammalian neurofilaments. Localization of...

    • researchdata.ands.org.au
    Published Jun 26, 2012
  88. o

    Data from: The secretome and N-glycosylation profiles of the charophycean...

    • www.omicsdi.org
  89. a

    Data from: Recombinant MUC1 probe authentically reflects cell-specific...

    • researchdata.ands.org.au
    Published Jun 26, 2012
  90. R

    LMAN family proteins bind glycosylated cargo

    • reactome.org
  91. a

    Data from: Glycosylation of eukaryotic peptide chain initiation factor 2...

    • researchdata.ands.org.au
    Published Jun 26, 2012
  92. o

    To understand how glycosylation on human B cells is regulated during...

    • omictools.com
    • datamed.org
    Updated Apr 20, 2017
  93. a

    Data from: Site-specific N-glycosylation of chicken serum IgG.

    • researchdata.ands.org.au
    Published Jun 26, 2012
  94. f

    Data from: Multiple Reaction Monitoring for the Quantitation of Serum...

    • acs.figshare.com
    Updated Dec 18, 2017
  95. a

    Data from: Alteration of asparagine-linked glycosylation in serum...

    • researchdata.ands.org.au
    Published Jun 26, 2012
  96. a

    Data from: Site-specific N-glycosylation of human chorionic...

    • researchdata.ands.org.au
    Published Jun 26, 2012
  97. o

    TAILS N-Terminomics and Proteomics Show Complex Regulation of Proteolytic...

    • www.omicsdi.org
  98. f

    Data from: Parallel Comparison of N‑Linked Glycopeptide Enrichment...

    • figshare.com
    Updated Feb 15, 2017
  99. o

    Data from: N-glycosylation site occupancy in human prostaglandin H synthases...

    • www.omicsdi.org
  100. Defective MOGS does not cleave glucose from an N-glycosylated protein

    • reactome.org
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Asparagine N-linked glycosylation

280 scholarly articles cite this dataset (View in Google Scholar)
Authors
Giovanni Dall'Olio
Available download formats from providers
sbgn, biopax2, pdf, biopax3, owl, sbml, docx
Description

N-linked glycosylation is the most important form of post-translational modification for proteins synthesized and folded in the Endoplasmic Reticulum (Stanley et al. 2009). An early study in 1999 revealed that about 50% of the proteins in the Swiss-Prot database at the time were N-glycosylated (Apweiler et al. 1999). It is now established that the majority of the proteins in the secretory pathway require glycosylation in order to achieve proper folding.
The addition of an N-glycan to a protein can have several roles (Shental-Bechor & Levy 2009). First, glycans enhance the solubility and stability of the proteins in the ER, the golgi and on the outside of the cell membrane, where the composition of the medium is strongly hydrophilic and where proteins, that are mostly hydrophobic, have difficulty folding properly. Second, N-glycans are used as signal molecules during the folding and transport process of the protein: they have the role of labels to determine when a protein must interact with a chaperon, be transported to the golgi, or targeted for degradation in case of major folding defects. Third, and most importantly, N-glycans on completely folded proteins are involved in a wide range of processes: they help determine the specificity of membrane receptors in innate immunity or in cell-to-cell interactions, they can change the properties of hormones and secreted proteins, or of the proteins in the vesicular system inside the cell.
All N-linked glycans are derived from a common 14-sugar oligosaccharide synthesized in the ER, which is attached co-translationally to a protein while this is being translated inside the reticulum. The process of the synthesis of this glycan, known as Synthesis of the N-glycan precursor or LLO, constitutes one of the most conserved pathways in eukaryotes, and has been also observed in some eubacteria. The attachment usually happens on an asparagine residue within the consensus sequence asparagine-X-threonine by an complex called oligosaccharyl transferase (OST).
After being attached to an unfolded protein, the glycan is used as a label molecule in the folding process (also known as Calnexin/Calreticulin cycle) (Lederkremer 2009). The majority of the glycoproteins in the ER require at least one glycosylated residue in order to achieve proper folding, even if it has been shown that a smaller portion of the proteins in the ER can be folded without this modification.
Once the glycoprotein has achieved proper folding, it is transported via the cis-Golgi through all the Golgi compartments, where the glycan is further modified according to the properties of the glycoprotein. This process involves relatively few enzymes but due to its combinatorial nature, can lead to several millions of different possible modifications. The exact topography of this network of reactions has not been established yet, representing one of the major challenges after the sequencing of the human genome (Hossler et al. 2006).
Since N-glycosylation is involved in an great number of different processes, from cell-cell interaction to folding control, mutations in one of the genes involved in glycan assembly and/or modification can lead to severe development problems (often affecting the central nervous system). All the diseases in genes involved in glycosylation are collectively known as Congenital Disorders of Glycosylation (CDG) (Sparks et al. 2003), and classified as CDG type I for the genes in the LLO synthesis pathway, and CDG type II for the others.

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